《植物生理学报》 2018, 54(5): 827-836

藏红花柱头小RNA高通量测序及生物信息学分析

卢晓慧^1,2, 朱志明³, 岳俊阳^2,4,*

¹嘉兴职业技术学院信息技术分院, 浙江嘉兴314000; ²安徽农业大学园艺学院, 合肥230036; ³嘉兴天禾藏红花专业合作社, 浙江嘉兴314000; ⁴合肥工业大学食品科学与工程学院, 合肥230009

通信作者：岳俊阳；E-mail: aaran.yue@gmail.com

摘　要：

通过构建藏红花开花前2 d与开花日两个不同时期柱头的小RNA文库并进行高通量测序, 分别获得3 711 809和2 303 043条序列。借助生物信息学的方法, 共鉴定出164条保守的miRNA。其中, 随开花过程下调表达和上调表达的miRNA分别是71和46条。靶基因预测及功能富集分析显示它们可能参与了硫辛酸生物合成、细胞程序性凋亡、花粉细胞的分化、脱落酸介导的信号途径等生物过程。本文筛选的藏红花成熟过程中的miRNA为今后脱辅基类胡萝卜素代谢调控网络的研究提供了可能的线索。

关键词：藏红花; 小RNA; miRNA; 高通量测序; 生物信息学

收稿：2018-01-08   修定：2018-04-26

资助：嘉兴科技公益性技术项目(2017AY33032)。

Bioinformatics analysis for high-throughput sequencing of small RNAs in the stigmas of saffron (Crocus sativus)

LU Xiao-Hui^1,2, ZHU Zhi-Ming³, YUE Jun-Yang^2,4,*

¹College of Information Technology, Jiaxing Vocational Technical College, Jiaxing, Zhejiang 314000, China; ²College of Horticulture, Anhui Agricultural University, Hefei 230036, China; ³Jiaxing Tianhe Saffron Specialized Cooperative, Jiaxing, Zhejiang 314000, China; ⁴School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China

Corresponding author: YUE Jun-Yang; E-mail: aaran.yue@gmail.com

Abstract:

In the present study, we employed the high-throughput sequencing technology to profile miRNAs in saffron stigmas from two different stages (2 days before anthesis and day of anthesis), with a total of 3 711 809 and 2 303 043 small RNA tags obtained, respectively. Bioinformatics analysis identified 164 conserved miRNAs, among which 71 miRNAs were down-regulated and 46 miRNAs were up-regulated. Furthermore, the potential target genes of these conserved miRNAs were predicted and subjected to gene ontology (GO) annotation. Enrichment analysis of the GO-represented biological processes revealed that these target genes were potentially involved in lipoic acid biosynthetic process, positive regulation of programmed cell death, pollen sperm cell differentiation, and positive regulation of abscisic acid mediated signaling. This study provides a useful resource for further elucidation of the regulatory roles of miRNAs that participate in apocarotenoid biosynthesis during saffron stigma development.

Key words: saffron; small RNA; miRNA; high-throughput sequencing; bioinformatics